Hotspot

ycf1-ndhF

Genome · 111367-111413 · 47 bp

Back to Ligusticopsis

Pi 0.0996

MarkerSeek score 63.70

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: Genome
length_bp: 47
variable_sites: 14
pi: 0.0996
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.57
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	CTCATGAAGGCCTACCGTTG	TGTTCGTTGGATCCCTAGGA	55.0	50.0	58.3	57.8	637-708 bp	1.0	83.9
ycf1-ndhF_p2	CCCTGCCCACACTAGAAAAA	GTGGGCGCATCTCTTCTTAT	50.0	50.0	57.7	57.8	838-909 bp	1.0	82.6
ycf1-ndhF_p3	CCCTGCCCACACTAGAAAAA	GGGGAATAAGAGGATTGGCC	50.0	55.0	57.7	57.7	958-1029 bp	1.0	82.2
ycf1-ndhF_p4	CCCTGCCCACACTAGAAAAA	GGGGGAATAAGAGGATTGGC	50.0	55.0	57.7	57.7	959-1030 bp	1.0	82.2
ycf1-ndhF_p5	CCCTGCCCACACTAGAAAAA	TGTTCGTTGGATCCCTAGGA	50.0	50.0	57.7	57.8	1312-1383 bp	1.0	81.4