Hotspot

trnL-CAA-rps7

Genome · 96765-97249 · 485 bp

Back to Ligusticopsis

Pi 0.0705

MarkerSeek score 64.20

Primer available yes

Label trnL-CAA-rps7

Feature Metadata

label_name: trnL-CAA-rps7
feature_type: igs
region: Genome
length_bp: 485
variable_sites: 75
pi: 0.0705
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-rps7_p1	AAATGGTAGACACGCGAGAC	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.0	58.0	592-593 bp	0.214286	48.6
trnL-CAA-rps7_p2	TGAAATGGTAGACACGCGAG	ATGGCAGAGGCAAATAGAGC	50.0	50.0	58.0	58.0	594-595 bp	0.214286	48.6
trnL-CAA-rps7_p3	AAATGGTAGACACGCGAGAC	TTGGTTATTAGCGGCATCCC	50.0	50.0	58.0	58.0	716-717 bp	0.214286	48.5
trnL-CAA-rps7_p4	TGAAATGGTAGACACGCGAG	TTGGTTATTAGCGGCATCCC	50.0	50.0	58.0	58.0	718-719 bp	0.214286	48.5
trnL-CAA-rps7_p5	AAATGGTAGACACGCGAGAC	TCCGAATTAGTGGATGCTGC	50.0	50.0	58.0	58.0	658-659 bp	0.214286	48.4