Hotspot

ycf1

SSC · 121808-126901 · 5094 bp

Back to Ligularia

Pi 0.00234

MarkerSeek score 40.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5094
variable_sites: 51
pi: 0.00234
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCTGTGCAATTTTCTATCGAAGT	ACAAAAACTAGATTGTCTAATGCTGA	34.8	30.8	56.5	57.4	200 bp	1.0	42.4
ycf1_p2	TGTTTCTGTGCAATTTTCTATCGA	ACAAAAACTAGATTGTCTAATGCTGA	33.3	30.8	57.4	57.4	204 bp	1.0	42.4
ycf1_p3	TCTGTGCAATTTTCTATCGAAGT	ACAAAAACTAGATTGTCTAATGCTG	34.8	32.0	56.5	56.1	200 bp	1.0	42.4
ycf1_p4	TCTGTGCAATTTTCTATCGAAGTT	ACAAAAACTAGATTGTCTAATGCTGA	33.3	30.8	57.2	57.4	200 bp	1.0	42.4
ycf1_p5	CAGATGTTTCTGTGCAATTTTCT	ACAAAAACTAGATTGTCTAATGCTGA	34.8	30.8	56.3	57.4	208 bp	1.0	42.3