Hotspot

rpoC1

LSC · 16074-18895 · 2822 bp

Back to Ligularia

Pi 0.000866

MarkerSeek score 51.10

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2822
variable_sites: 8
pi: 0.000866
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	TCTGAGAAGACTTTCCAGCT	GTACCTCCATATTGCGCTGA	45.0	50.0	55.8	57.5	2966-2984 bp	1.0	58.7
rpoC1_p2	TCTGAGAAGACTTTCCAGCT	TCAGTACCTCCATATTGCGC	45.0	50.0	55.8	57.5	2969-2987 bp	1.0	58.7
rpoC1_p3	TCTGAGAAGACTTTCCAGCT	AGTACCTCCATATTGCGCTG	45.0	50.0	55.8	57.5	2967-2985 bp	1.0	58.7
rpoC1_p4	TCTGAGAAGACTTTCCAGCT	CAGTACCTCCATATTGCGCT	45.0	50.0	55.8	57.5	2968-2986 bp	1.0	58.7
rpoC1_p5	TTCGGTTGTTCGTTCGAGAA	GGTTTGAGTCAGTGATTGGA	45.0	45.0	57.8	55.3	2989-3000 bp	0.666667	43.7