Hotspot

rpl16

LSC · 80250-81685 · 1436 bp

Back to Ligularia

Pi 0.00275

MarkerSeek score 40.50

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1436
variable_sites: 12
pi: 0.00275
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2494-2515 bp	1.0	80.4
rpl16_p2	TCGACGATTGCTAGCTCCTA	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2284-2305 bp	1.0	80.3
rpl16_p3	CGCACCAAAAACTCGAGTTC	GCAAATTGCAGGACGTATCG	50.0	50.0	58.0	57.9	2177-2198 bp	1.0	80.3
rpl16_p4	GCGTCTTTGATCACAGCAAC	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2328-2349 bp	1.0	80.2
rpl16_p5	TCTCAATTCTCGGGCAATCG	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.1	58.1	2512-2533 bp	1.0	80.0