Hotspot

ndhA

SSC · 117895-120051 · 2157 bp

Back to Ligularia

Pi 0.00232
MarkerSeek score 43.10
Primer available yes
Label ndhA

Feature Metadata

label_name
ndhA
feature_type
gene
region
SSC
length_bp
2157
variable_sites
16
pi
0.00232
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhA_p1 TGTTTGAGACAAGTCGTGAA CCTCCGGGGTTTATTAATTTGC 40.0 45.5 55.2 57.7 2296-2311 bp 1.0 41.1
ndhA_p2 TTGTTTGAGACAAGTCGTGA CCTCCGGGGTTTATTAATTTGC 40.0 45.5 55.2 57.7 2297-2312 bp 1.0 41.1
ndhA_p3 TTGTTTGAGACAAGTCGTGAA CCTCCGGGGTTTATTAATTTGC 38.1 45.5 56.0 57.7 2297-2312 bp 1.0 41.1
ndhA_p4 TGTTTGAGACAAGTCGTGAAT CCTCCGGGGTTTATTAATTTGC 38.1 45.5 55.8 57.7 2296-2311 bp 1.0 41.1
ndhA_p5 TGTTTGAGACAAGTCGTGAA CTCCGGGGTTTATTAATTTGCA 40.0 40.9 55.2 57.0 2295-2310 bp 1.0 41.1