Hotspot

ndhG-ndhI

SSC · 116940-117315 · 376 bp

Back to Ligularia

Pi 0.00561

MarkerSeek score 38.30

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 376
variable_sites: 7
pi: 0.00561
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	AGACCACGGAAGTTACTCCA	TTGATCACATCCGAGCGTTT	50.0	45.0	58.0	57.9	1033-1049 bp	1.0	81.1
ndhG-ndhI_p2	AGACTTGTACAGACCACGGA	TTGATCACATCCGAGCGTTT	50.0	45.0	58.0	57.9	1043-1059 bp	1.0	81.1
ndhG-ndhI_p3	AGACCACGGAAGTTACTCCA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1368-1384 bp	1.0	81.0
ndhG-ndhI_p4	AGACTTGTACAGACCACGGA	GATGGACTTTACCGAGGCTG	50.0	55.0	58.0	58.1	1378-1394 bp	1.0	81.0
ndhG-ndhI_p5	AGACCACGGAAGTTACTCCA	AGATGGACTTTACCGAGGCT	50.0	50.0	58.0	58.1	1369-1385 bp	1.0	80.4