Hotspot

rpl32-trnL-UAG

SSC · 111545-112336 · 792 bp

Back to Ligularia

Pi 0.00505
MarkerSeek score 47.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
792
variable_sites
13
pi
0.00505
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGCAGCGTTAAAAGCTTTGT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 836-910 bp 1.0 84.1
rpl32-trnL-UAG_p2 GGCAGCGTTAAAAGCTTTGT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 837-911 bp 1.0 84.1
rpl32-trnL-UAG_p3 AAAGGATATTGGGCAGCGTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 847-921 bp 1.0 84.1
rpl32-trnL-UAG_p4 AAAGGATATTGGGCAGCGTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 848-922 bp 1.0 84.1
rpl32-trnL-UAG_p5 GGCAGCGTTAAAAGCTTTGT GAAGTAGCAAAGCCTTGGGT 45.0 50.0 57.9 58.1 1350-1427 bp 1.0 83.2