Hotspot

accD-psaI

LSC · 58203-58868 · 666 bp

Back to Ligularia

Pi 0.00344

MarkerSeek score 48.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 666
variable_sites: 7
pi: 0.00344
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	800-810 bp	1.0	63.0
accD-psaI_p2	AAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	799-809 bp	1.0	63.0
accD-psaI_p3	TCAACTCCACACTTTCTTTCCT	AAAGAAGCCATTGCGATTGC	40.9	45.0	57.5	58.0	776-786 bp	1.0	62.7
accD-psaI_p4	AAAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	38.1	45.0	56.3	58.0	800-810 bp	1.0	61.2
accD-psaI_p5	AAAAGCGTTCTGAGTGAGTT	TTGCGATTGCCGGAAATACT	40.0	45.0	55.5	58.3	789-799 bp	1.0	61.0