Hotspot

trnM-CAU-atpE

LSC · 51890-52092 · 203 bp

Back to Ligularia

Pi 0.0107

MarkerSeek score 48.30

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 203
variable_sites: 9
pi: 0.0107
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GCGGGAGTCATTGGTTCAAA	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.8	57.9	296 bp	1.0	75.4
trnM-CAU-atpE_p2	GCGGGAGTCATTGGTTCAAA	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.8	58.1	298 bp	1.0	75.2
trnM-CAU-atpE_p3	GCGGGAGTCATTGGTTCAAA	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.8	57.9	300 bp	1.0	75.2
trnM-CAU-atpE_p4	GCGGGAGTCATTGGTTCAAA	AGGCAAATCTAGCTCTCCGA	50.0	50.0	58.8	58.2	299 bp	1.0	74.6
trnM-CAU-atpE_p5	GCGGGAGTCATTGGTTCAAA	CTAGGACACGAGTAGAGGCT	50.0	55.0	58.8	57.7	275 bp	1.0	73.9