Hotspot

petN-psbM

LSC · 10342-10841 · 500 bp

Back to Ligularia

Pi 0.00356

MarkerSeek score 48.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 500
variable_sites: 5
pi: 0.00356
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.2	55.6	657-662 bp	1.0	60.6
petN-psbM_p2	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTTT	50.0	38.1	58.2	56.4	657-662 bp	1.0	58.9
petN-psbM_p3	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTT	50.0	40.0	57.1	55.6	658-663 bp	1.0	54.7
petN-psbM_p4	CTCGCTTGGGCTGCTTTAAT	TGCTACTGCACTGTTCATTT	50.0	40.0	58.9	55.6	649-654 bp	1.0	54.6
petN-psbM_p5	TGGGCTGCTTTAATGGTAGTC	TGCTACTGCACTGTTCATTT	47.6	40.0	58.0	55.6	643-648 bp	1.0	53.8