Hotspot

rpl16

LSC · 84216-85765 · 1550 bp

Back to Licuala

Pi 0.0122

MarkerSeek score 56.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1550
variable_sites: 41
pi: 0.0122
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	CGCACCAAAAACTCGAGTTC	ACCGGAAACTCAACATTGCT	50.0	45.0	58.0	58.0	2457-2547 bp	1.0	83.7
rpl16_p2	CCGCTGTTATCCGCTACATT	ACCGGAAACTCAACATTGCT	50.0	45.0	58.1	58.0	2198-2288 bp	1.0	83.2
rpl16_p3	AACTGTCTCAATTCCCGAGC	ACCGGAAACTCAACATTGCT	50.0	45.0	57.9	58.0	2480-2570 bp	1.0	83.0
rpl16_p4	GTCCAGAATAAACCTGCGGT	ACCGGAAACTCAACATTGCT	50.0	45.0	57.9	58.0	2091-2161 bp	1.0	82.9
rpl16_p5	ATCGCACCAAAAACTCGAGT	ACCGGAAACTCAACATTGCT	45.0	45.0	58.1	58.0	2459-2549 bp	1.0	82.9