Hotspot

psaC-ndhE

SSC · 120469-121299 · 831 bp

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Pi 0.0612

MarkerSeek score 75.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 831
variable_sites: 59
pi: 0.0612
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 75.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGTACACATTGAGTACACCCT	CTGAAGCAGCTATTGGGCTA	47.6	50.0	57.4	57.4	533-979 bp	1.0	72.8
psaC-ndhE_p2	CGTACACATTGAGTACACCCT	TGAAGCAGCTATTGGGCTAG	47.6	50.0	57.4	57.4	532-978 bp	1.0	72.8
psaC-ndhE_p3	CGTACACATTGAGTACACCCT	TGTTATAGCTGTTGCGGCTG	47.6	50.0	57.4	58.6	553-999 bp	1.0	72.6
psaC-ndhE_p4	CGTACACATTGAGTACACCCT	GAAGCAGCTATTGGGCTAGC	47.6	55.0	57.4	58.8	531-977 bp	1.0	71.7
psaC-ndhE_p5	CGTACACATTGAGTACACCCT	TTTGTTATAGCTGTTGCGGC	47.6	45.0	57.4	57.1	555-1001 bp	1.0	70.3