Hotspot

rps12-trnV-GAC

IRb · 101628-103500 · 1873 bp

Back to Licuala

Pi 0.000964
MarkerSeek score 59.20
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
1873
variable_sites
4
pi
0.000964
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.99
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 TGGCAACTTTACGTAAGGCA CGGGGGAGCAAGTCAAAATA 45.0 50.0 57.7 57.9 2020-2030 bp 1.0 77.5
rps12-trnV-GAC_p2 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1959-1969 bp 1.0 77.2
rps12-trnV-GAC_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1958-1968 bp 1.0 77.2
rps12-trnV-GAC_p4 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1963-1973 bp 1.0 77.1
rps12-trnV-GAC_p5 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1961-1971 bp 1.0 76.2