Hotspot

rpl22-rps19

IRb · 87041-87131 · 91 bp

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Pi 0.0462

MarkerSeek score 87.10

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: IRb
length_bp: 91
variable_sites: 10
pi: 0.0462
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTCCGCGAATCTGATCAAT	TCTGACTTTCGCGAGACATG	50.0	50.0	58.5	58.0	255-263 bp	1.0	79.2
rpl22-rps19_p2	CGTCCGCGAATCTGATCAAT	CCTACTCTGACTTTCGCGAG	50.0	55.0	58.5	57.8	260-268 bp	1.0	77.8
rpl22-rps19_p3	CGTCCGCGAATCTGATCAAT	CTCTGACTTTCGCGAGACAT	50.0	50.0	58.5	57.7	256-264 bp	1.0	77.2
rpl22-rps19_p4	CGTCCGCGAATCTGATCAAT	CAAATTGGGAGAATTCGCGC	50.0	50.0	58.5	58.5	279-287 bp	1.0	75.5
rpl22-rps19_p5	CGTCCGCGAATCTGATCAAT	TTCGCGAGACATGCAAGAAA	50.0	45.0	58.5	58.5	248-256 bp	1.0	75.5