Hotspot

petA-psbJ

LSC · 65431-66437 · 1007 bp

Back to Licuala

Pi 0.0185

MarkerSeek score 57.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1007
variable_sites: 34
pi: 0.0185
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	ATCCATTACGTGTCCAAGGC	GGGTTGGGTTCATCTCTGTAG	50.0	52.4	58.0	57.7	1147-1160 bp	1.0	71.9
petA-psbJ_p2	GGCCTTTTGTTCTTCTTGGC	GGGTTGGGTTCATCTCTGTAG	50.0	52.4	57.8	57.7	1130-1143 bp	1.0	71.0
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GGGTTGGGTTCATCTCTGTAG	50.0	52.4	58.2	57.7	1144-1157 bp	1.0	70.8
petA-psbJ_p4	ATCCATTACGTGTCCAAGGC	GGGTTGGGTTCATCTCTGTA	50.0	50.0	58.0	56.5	1147-1160 bp	1.0	70.3
petA-psbJ_p5	TTACGTGTCCAAGGCCTTTT	GGGTTGGGTTCATCTCTGTAG	45.0	52.4	57.6	57.7	1142-1155 bp	1.0	69.4