Hotspot

accD-psaI

LSC · 61444-62323 · 880 bp

Back to Licuala

Pi 0.0195

MarkerSeek score 63.30

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 880
variable_sites: 34
pi: 0.0195
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CCACGGTTTCTTTCCCTTGA	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	959-1165 bp	1.0	86.6
accD-psaI_p2	CCACGGTTTCTTTCCCTTGA	TGGTCCCATCGGATCTAGAC	50.0	55.0	58.0	58.0	960-1166 bp	1.0	86.6
accD-psaI_p3	TCCACGGTTTCTTTCCCTTG	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	960-1166 bp	1.0	86.6
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	TGGTCCCATCGGATCTAGAC	50.0	55.0	58.0	58.0	961-1167 bp	1.0	86.6
accD-psaI_p5	GCGAGAAAATCACCCGTTTG	GGTCCCATCGGATCTAGACA	50.0	55.0	58.0	58.0	1374-1580 bp	1.0	86.0