Hotspot

psaA-ycf3

LSC · 42902-43554 · 653 bp

Back to Licuala

Pi 0.00347

MarkerSeek score 59.70

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 5
pi: 0.00347
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	TTGAAGATCACGAGGCGTTT	50.0	45.0	57.9	57.8	739-767 bp	1.0	81.9
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TTGGTTGAAGATCACGAGGC	50.0	50.0	57.9	58.2	743-771 bp	1.0	81.7
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	ATCACGAGGCGTTTCGAATT	50.0	45.0	57.9	58.3	733-761 bp	1.0	81.1
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	TGAAGATCACGAGGCGTTTC	50.0	50.0	57.9	58.3	738-766 bp	1.0	80.9
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	815-843 bp	1.0	80.6