Hotspot

petN-psbM

LSC · 30005-30825 · 821 bp

Back to Licuala

Pi 0.0257

MarkerSeek score 71.00

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 821
variable_sites: 41
pi: 0.0257
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	GTCTCACTTGGGCTGCTTTA	TGTTCATTCTAGTTCCTACTGCT	50.0	39.1	57.8	57.0	875-1060 bp	1.0	53.9
petN-psbM_p2	GTCTCACTTGGGCTGCTTTA	CTGTTCATTCTAGTTCCTACTGCT	50.0	41.7	57.8	58.0	876-1061 bp	1.0	53.7
petN-psbM_p3	ACTTGGGCTGCTTTAATGGT	TGTTCATTCTAGTTCCTACTGCT	45.0	39.1	57.7	57.0	870-1055 bp	1.0	52.9
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGTTCATTCTAGTTCCTACTGCT	50.0	39.1	57.6	57.0	820-1005 bp	1.0	52.8
petN-psbM_p5	ACTTGGGCTGCTTTAATGGT	CTGTTCATTCTAGTTCCTACTGCT	45.0	41.7	57.7	58.0	871-1056 bp	1.0	52.7