Hotspot

trnR-UCU-atpA

LSC · 10485-10583 · 99 bp

Back to Licuala

Pi 0.0152

MarkerSeek score 58.30

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 99
variable_sites: 3
pi: 0.0152
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AAGCTCTTTTGAGGGAAGCT	47.6	45.0	57.3	57.0	200-207 bp	1.0	62.7
trnR-UCU-atpA_p2	CGAAAAGCGTCCATTGTCTA	AAGCTCTTTTGAGGGAAGCT	45.0	45.0	56.2	57.0	256-263 bp	1.0	60.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	GAAGCTCTTTTGAGGGAAGCT	47.6	47.6	57.3	58.2	201-208 bp	1.0	60.7
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	GAAGCTCTTTTGAGGGAAGC	47.6	50.0	57.3	56.7	201-208 bp	1.0	60.1
trnR-UCU-atpA_p5	TGGATAGGACAGAGGTCTTCT	AAGCTCTTTTGAGGGAAGCT	47.6	45.0	56.9	57.0	235-242 bp	1.0	59.2