Hotspot

trnS-GCU-trnG-UCC

LSC · 8589-9445 · 857 bp

Back to Licuala

Pi 0.0524

MarkerSeek score 74.50

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 857
variable_sites: 55
pi: 0.0524
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	ATTAGCAATCCGCCGCTTTA	ACGAATCACACTTTTACCACT	45.0	38.1	58.0	55.5	546-931 bp	1.0	61.6
trnS-GCU-trnG-UCC_p2	ATTAGCAATCCGCCGCTTTA	AGAACGAATCACACTTTTACCAC	45.0	39.1	58.0	57.3	549-934 bp	1.0	60.5
trnS-GCU-trnG-UCC_p3	ATTAGCAATCCGCCGCTTTA	GAACGAATCACACTTTTACCACT	45.0	39.1	58.0	57.3	548-933 bp	1.0	60.5
trnS-GCU-trnG-UCC_p4	ATTAGCAATCCGCCGCTTTA	AACGAATCACACTTTTACCACT	45.0	36.4	58.0	56.2	547-932 bp	1.0	59.7
trnS-GCU-trnG-UCC_p5	GCTTTAGTCCACTCAGCCAT	ACGAATCACACTTTTACCACT	50.0	38.1	57.6	55.5	532-917 bp	1.0	58.7