Hotspot

rpl16

LSC · 85889-87393 · 1505 bp

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Pi 0.00589

MarkerSeek score 59.70

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1505
variable_sites: 20
pi: 0.00589
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GGACCTTTTCCTTTGGTTTTTCT	ACTCTTTCATTCCTCTTTGTCT	39.1	36.4	57.6	55.0	1619-1652 bp	1.0	43.4
rpl16_p2	GGACCTTTTCCTTTGGTTTTTCT	TCCTCTTTGTCTTCAATCAAAACA	39.1	33.3	57.6	57.0	1609-1642 bp	1.0	43.4
rpl16_p3	GGACCTTTTCCTTTGGTTTTTCT	ACAAACTCTTTCATTCCTCTTTGT	39.1	33.3	57.6	57.0	1623-1656 bp	1.0	43.4
rpl16_p4	GGACCTTTTCCTTTGGTTTTTCT	ACAAACTCTTTCATTCCTCTTTGTC	39.1	36.0	57.6	58.0	1623-1656 bp	1.0	43.4
rpl16_p5	GGACCTTTTCCTTTGGTTTTTCT	CTCTTTCATTCCTCTTTGTCTTCA	39.1	37.5	57.6	56.9	1618-1651 bp	1.0	43.4