Hotspot

trnR-ACG-rrn5

IRa · 137823-138096 · 274 bp

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Pi 0.00656

MarkerSeek score 34.90

Primer available yes

Label trnR-ACG-rrn5

Feature Metadata

label_name: trnR-ACG-rrn5
feature_type: igs
region: IRa
length_bp: 274
variable_sites: 3
pi: 0.00656
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-rrn5_p1	CCACGTGCTCTAATCCTCTG	CCTGCGGAAAAATAGCTCGA	55.0	50.0	57.8	58.3	314-335 bp	1.0	80.2
trnR-ACG-rrn5_p2	CCACGTGCTCTAATCCTCTG	TCCTGCGGAAAAATAGCTCG	55.0	50.0	57.8	58.3	315-336 bp	1.0	80.2
trnR-ACG-rrn5_p3	CCACGTGCTCTAATCCTCTG	AAAAATAGCTCGACGCCAGG	55.0	50.0	57.8	58.6	307-328 bp	1.0	78.0
trnR-ACG-rrn5_p4	CCACGTGCTCTAATCCTCTG	GGAAAAATAGCTCGACGCCA	55.0	50.0	57.8	58.6	309-330 bp	1.0	78.0
trnR-ACG-rrn5_p5	CCACGTGCTCTAATCCTCTG	GAAAAATAGCTCGACGCCAG	55.0	50.0	57.8	57.3	308-329 bp	1.0	77.3