Hotspot

rpl32-trnL-UAG

SSC · 119467-120752 · 1286 bp

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Pi 0.00376
MarkerSeek score 63.40
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1286
variable_sites
13
pi
0.00376
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACCGCGTTGAAAGCTTTTTC AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1411-1448 bp 1.0 80.4
rpl32-trnL-UAG_p2 GACCGCGTTGAAAGCTTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 58.2 58.0 1412-1449 bp 1.0 80.4
rpl32-trnL-UAG_p3 ACCGCGTTGAAAGCTTTTTC CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1412-1449 bp 1.0 80.4
rpl32-trnL-UAG_p4 GACCGCGTTGAAAGCTTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 58.2 58.0 1413-1450 bp 1.0 80.4
rpl32-trnL-UAG_p5 ACCGCGTTGAAAGCTTTTTC TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 1421-1458 bp 1.0 79.5