Hotspot

ndhF-rpl32

SSC · 118228-119292 · 1065 bp

Back to Licania

Pi 0.00764
MarkerSeek score 56.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1065
variable_sites
20
pi
0.00764
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGCAAGTGTAACGAAGGGT AAAAAGCTTTCAACGCGGTC 50.0 45.0 58.1 58.2 1213-1237 bp 1.0 79.7
ndhF-rpl32_p2 CTGCAAGTGTAACGAAGGGT GAAAAAGCTTTCAACGCGGT 50.0 45.0 58.1 58.2 1214-1238 bp 1.0 79.7
ndhF-rpl32_p3 ACGAAGGGTATGATCCACGA AAAAAGCTTTCAACGCGGTC 50.0 45.0 58.2 58.2 1202-1226 bp 1.0 78.8
ndhF-rpl32_p4 ACGAAGGGTATGATCCACGA GAAAAAGCTTTCAACGCGGT 50.0 45.0 58.2 58.2 1203-1227 bp 1.0 78.8
ndhF-rpl32_p5 CTGCAAGTGTAACGAAGGGT TTCAACGCGGTCCAATATCC 50.0 50.0 58.1 58.3 1205-1229 bp 1.0 78.3