Hotspot

rrn4.5-rrn5

IRb · 112951-113177 · 227 bp

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Pi 0.0206

MarkerSeek score 45.60

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 227
variable_sites: 14
pi: 0.0206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AAGTTCGGGATGGATTGGTG	55.0	50.0	58.0	57.9	313 bp	1.0	82.2
rrn4.5-rrn5_p2	GTCAAGTGGAAGTGCAGTGA	AAGTTCGGGATGGATTGGTG	50.0	50.0	58.0	57.9	339 bp	1.0	81.9
rrn4.5-rrn5_p3	GTCAAGTGGAAGTGCAGTGA	ACCAAGTTCGGGATGGATTG	50.0	50.0	58.0	57.9	342 bp	1.0	81.9
rrn4.5-rrn5_p4	GTCAAGTGGAAGTGCAGTGA	CAAGTTCGGGATGGATTGGT	50.0	50.0	58.0	57.9	340 bp	1.0	81.9
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	CACCAAGTTCGGGATGGATT	50.0	50.0	58.0	57.9	343 bp	1.0	81.9