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psaJ-rpl33

LSC · 71033-71543 · 511 bp

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Pi 0.0138

MarkerSeek score 48.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 511
variable_sites: 16
pi: 0.0138
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCGTTGACATTCCCGTTTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	57.9	58.2	562-586 bp	1.0	79.6
psaJ-rpl33_p2	GCGTTGACATTCCCGTTTTT	GACATCTTTACCCTTGGCCA	45.0	50.0	57.9	57.5	561-585 bp	1.0	77.5
psaJ-rpl33_p3	GCGTTGACATTCCCGTTTTT	CGGACATCTTTACCCTTGGC	45.0	55.0	57.9	58.6	563-587 bp	1.0	76.5
psaJ-rpl33_p4	ATGCGTTGACATTCCCGTTT	GGACATCTTTACCCTTGGCC	45.0	55.0	58.8	58.2	564-588 bp	1.0	74.6
psaJ-rpl33_p5	GCGTTGACATTCCCGTTTTT	GACATCTTTACCCTTGGCCAT	45.0	47.6	57.9	58.0	561-585 bp	1.0	73.4