Hotspot

trnF-GAA-ndhJ

LSC · 50474-51295 · 822 bp

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Pi 0.00762

MarkerSeek score 53.40

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 822
variable_sites: 16
pi: 0.00762
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GAAAGTTGGATAGGGTGGCC	55.0	55.0	58.0	58.2	944-1019 bp	1.0	81.3
trnF-GAA-ndhJ_p2	GCATCAGAAATGGTCGGGAT	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.0	58.2	959-1034 bp	1.0	81.0
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.1	58.2	908-983 bp	1.0	81.0
trnF-GAA-ndhJ_p4	CTGGTAGAGCAGAGGACTGA	GAAAGTTGGATAGGGTGGCC	55.0	55.0	57.9	58.2	932-1007 bp	1.0	80.2
trnF-GAA-ndhJ_p5	ATGCATCAGAAATGGTCGGG	GAAAGTTGGATAGGGTGGCC	50.0	55.0	58.3	58.2	961-1036 bp	1.0	78.7