Hotspot

trnG-UCC-trnfM-CAU

LSC · 39167-39367 · 201 bp

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Pi 0.0124

MarkerSeek score 56.20

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 201
variable_sites: 5
pi: 0.0124
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	TCTTTGCCAAGGAGAAGACG	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.8	58.0	272-281 bp	1.0	81.8
trnG-UCC-trnfM-CAU_p2	TCTTTGCCAAGGAGAAGACG	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.8	57.8	270-279 bp	1.0	80.7
trnG-UCC-trnfM-CAU_p3	TCTTTGCCAAGGAGAAGACG	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.8	57.8	293-302 bp	1.0	80.1
trnG-UCC-trnfM-CAU_p4	TCTTTGCCAAGGAGAAGACG	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.8	57.8	292-301 bp	1.0	80.1
trnG-UCC-trnfM-CAU_p5	TCTTTGCCAAGGAGAAGACG	GTTCTTCTCGCGGAGTAGAG	50.0	55.0	57.8	57.5	327 bp	0.166667	41.6