Hotspot

trnT-GGU-psbD

LSC · 33503-35096 · 1594 bp

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Pi 0.00318

MarkerSeek score 62.30

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1594
variable_sites: 15
pi: 0.00318
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	TATGTTGTCCGCATAGTGGC	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1773-1802 bp	1.0	81.2
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	GAAACGGTCCCTACGTAACC	55.0	55.0	58.1	58.0	1715-1744 bp	1.0	81.1
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	GAAACGGTCCCTACGTAACC	50.0	55.0	58.1	58.0	1718-1747 bp	1.0	80.9
trnT-GGU-psbD_p4	TATGTTGTCCGCATAGTGGC	AAGCAATAGACCGGACCAAC	50.0	50.0	58.1	57.9	1803-1832 bp	1.0	80.3
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	AAGCAATAGACCGGACCAAC	55.0	50.0	58.1	57.9	1745-1774 bp	1.0	80.3