Hotspot

rpoB-trnC-GCA

LSC · 27286-28585 · 1300 bp

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Pi 0.00511

MarkerSeek score 45.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1300
variable_sites: 17
pi: 0.00511
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 45.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTGCAGTCCC	CTTACCGCTCGGCCATGT	50.0	61.1	57.9	59.8	1339-1348 bp	1.0	49.8
rpoB-trnC-GCA_p2	ATTCCCTCATTTGCAGTCCC	TTACCGCTCGGCCATGTC	50.0	61.1	57.9	59.8	1338-1347 bp	1.0	49.8
rpoB-trnC-GCA_p3	ATTCCCTCATTTGCAGTCCC	ATTTGCAGTCCCCCGCCTTA	50.0	55.0	57.9	61.9	1355-1364 bp	1.0	49.5
rpoB-trnC-GCA_p4	ATTCCCTCATTTGCAGTCCC	CTTACCGCTCGGCCATGTC	50.0	63.2	57.9	60.9	1339-1348 bp	1.0	49.3
rpoB-trnC-GCA_p5	GCCCTGACCAATGAACCTAC	CTTACCGCTCGGCCATGT	55.0	61.1	58.2	59.8	1403-1412 bp	1.0	49.0