Hotspot

trnR-UCU-atpA

LSC · 9860-10106 · 247 bp

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Pi 0.0408

MarkerSeek score 74.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 247
variable_sites: 16
pi: 0.0408
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAAAAGCGTCCATTGTCT	ATTCGGGAACAGATGGAACG	45.0	50.0	57.7	58.0	361-411 bp	1.0	84.9
trnR-UCU-atpA_p2	TGGAAAAGCGTCCATTGTCT	CGGGAACAGATGGAACGTTT	45.0	50.0	57.7	58.5	358-408 bp	1.0	81.3
trnR-UCU-atpA_p3	TTTGGAATGGAAAAGCGTCC	ATTCGGGAACAGATGGAACG	45.0	50.0	56.9	58.0	368-418 bp	1.0	78.3
trnR-UCU-atpA_p4	TGGAAAAGCGTCCATTGTCT	AAACATTCACCGAAGAGGCG	45.0	50.0	57.7	58.8	402-452 bp	1.0	77.8
trnR-UCU-atpA_p5	TGGAAAAGCGTCCATTGTCT	GCTATTCGGGAACAGATGGA	45.0	50.0	57.7	57.1	364-414 bp	1.0	77.8