Hotspot

trnK-UUU-trnQ-UUG

LSC · 4464-5896 · 1433 bp

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Pi 0.00899

MarkerSeek score 60.60

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1433
variable_sites: 24
pi: 0.00899
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	AAAGCCGAGTACTCTACCGT	TGAATAGAAATGGGGCGTGG	50.0	50.0	58.2	57.9	1514-1554 bp	1.0	81.4
trnK-UUU-trnQ-UUG_p2	AAGCCGAGTACTCTACCGTT	TGAATAGAAATGGGGCGTGG	50.0	50.0	58.2	57.9	1513-1553 bp	1.0	81.4
trnK-UUU-trnQ-UUG_p3	AAAGCCGAGTACTCTACCGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1456-1496 bp	1.0	81.3
trnK-UUU-trnQ-UUG_p4	AAGCCGAGTACTCTACCGTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1455-1495 bp	1.0	81.3
trnK-UUU-trnQ-UUG_p5	TACCGTTGAGTTAGCAACCC	TGAATAGAAATGGGGCGTGG	50.0	50.0	57.5	57.9	1500-1540 bp	1.0	79.1