Hotspot

rpl16

LSC · 84390-86019 · 1630 bp

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Pi 0.0101

MarkerSeek score 56.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1630
variable_sites: 16
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TGACAACCGCAGCATTATCA	CGAGGGAATTGCACGGATAA	45.0	50.0	57.9	58.1	2594-2642 bp	1.0	81.0
rpl16_p2	ACCTCGGGTGCTAATGAAAC	CGAGGGAATTGCACGGATAA	50.0	50.0	57.9	58.1	2697-2745 bp	1.0	80.9
rpl16_p3	TGACAACCGCAGCATTATCA	TCTTCTGGTGTCGAGGGAAT	45.0	50.0	57.9	58.1	2605-2653 bp	1.0	80.9
rpl16_p4	CCGCTGTTATCGGCTACATT	CGAGGGAATTGCACGGATAA	50.0	50.0	58.1	58.1	2379-2427 bp	1.0	80.9
rpl16_p5	ACCTCGGGTGCTAATGAAAC	TCTTCTGGTGTCGAGGGAAT	50.0	50.0	57.9	58.1	2708-2756 bp	1.0	80.8