Hotspot

ccsA

SSC · 117658-118629 · 972 bp

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Pi 0.00206

MarkerSeek score 47.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 2
pi: 0.00206
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGGATATTGGGCAGCATTGA	CAGTTGACAAGGTCGAAGCT	50.0	50.0	58.0	58.1	1952-1981 bp	1.0	81.9
ccsA_p2	GGGATATTGGGCAGCATTGA	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1792 bp	0.5	59.5
ccsA_p3	AGGGATATTGGGCAGCATTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1793 bp	0.5	59.4
ccsA_p4	GTGAAATCGGTAGACACGCT	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1309 bp	0.5	59.4
ccsA_p5	TGAAATCGGTAGACACGCTG	GGTGTTTGTGAGAACCCCTT	50.0	50.0	58.0	57.9	1308 bp	0.5	59.4