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trnL-UAG-ccsA

SSC · 117530-117657 · 128 bp

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Pi 0.0408

MarkerSeek score 70.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 128
variable_sites: 4
pi: 0.0408
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATTGATACGACCGAAAACG	50.0	40.9	58.0	57.7	231-261 bp	1.0	66.7
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATTGATACGACCGAAAACG	50.0	40.9	58.0	57.7	230-260 bp	1.0	66.7
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	ACAATTGATACGACCGAAAACG	50.0	40.9	57.9	57.7	221-251 bp	1.0	65.8
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	ACAATTGATACGACCGAAAACG	55.0	40.9	57.8	57.7	223-253 bp	1.0	65.2
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	ACAATTGATACGACCGAAAACG	55.0	40.9	57.8	57.7	222-252 bp	1.0	65.2