Hotspot

rpl32-trnL-UAG

SSC · 117076-117449 · 374 bp

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Pi 0.0355

MarkerSeek score 68.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 374
variable_sites: 13
pi: 0.0355
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCATTGA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	500-503 bp	1.0	83.3
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCATTGA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	501-504 bp	1.0	83.3
rpl32-trnL-UAG_p3	AGGGATATTGGGCAGCATTG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	501-504 bp	1.0	83.2
rpl32-trnL-UAG_p4	AGGGATATTGGGCAGCATTG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	502-505 bp	1.0	83.2
rpl32-trnL-UAG_p5	GAGAAGGGATATTGGGCAGC	AGCGTGTCTACCGATTTCAC	55.0	50.0	58.1	58.0	505-508 bp	1.0	82.5