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rps8-rpl14

LSC · 83573-83874 · 302 bp

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Pi 0.0938

MarkerSeek score 86.90

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: LSC
length_bp: 302
variable_sites: 24
pi: 0.0938
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 86.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	GCAATGGTGTCCTTACCCAT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.9	57.9	304-349 bp	1.0	87.9
rps8-rpl14_p2	GATGGTATTCGAACCGTCCC	GTTTCATTAGCACCCGAGGT	55.0	50.0	58.1	57.9	367-412 bp	1.0	86.9
rps8-rpl14_p3	GCAATGGTGTCCTTACCCAT	AGTTTCATTAGCACCCGAGG	50.0	50.0	57.9	57.6	305-350 bp	1.0	85.5
rps8-rpl14_p4	GGTATTCGAACCGTCCCTTT	GTTTCATTAGCACCCGAGGT	50.0	50.0	57.6	57.9	364-409 bp	1.0	85.0
rps8-rpl14_p5	GATGGTATTCGAACCGTCCC	AGTTTCATTAGCACCCGAGG	55.0	50.0	58.1	57.6	368-413 bp	1.0	84.6