Hotspot

rpl33-rps18

LSC · 70618-70822 · 205 bp

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Pi 0.0248

MarkerSeek score 68.10

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 205
variable_sites: 5
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	TCTGTCCCTGTTGTTGCAAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	341-342 bp	1.0	78.7
rpl33-rps18_p2	TTCTGTCCCTGTTGTTGCAA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.9	57.7	342-343 bp	1.0	78.7
rpl33-rps18_p3	ATTCTGTCCCTGTTGTTGCA	TCAATTCGATCCCCCGATTG	45.0	50.0	57.6	57.7	343-344 bp	1.0	76.9
rpl33-rps18_p4	TCTGTCCCTGTTGTTGCAAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	338-339 bp	1.0	75.7
rpl33-rps18_p5	TTCTGTCCCTGTTGTTGCAA	ATTCGATCCCCCGATTGGAT	45.0	50.0	57.9	58.6	339-340 bp	1.0	75.7