Hotspot

psaJ-rpl33

LSC · 69898-70416 · 519 bp

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Pi 0.0175

MarkerSeek score 67.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 9
pi: 0.0175
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TACTCTATGGTTCGGGGCTT	CACTGTTTCGAACACAACTGG	50.0	47.6	57.8	57.9	674-677 bp	1.0	71.8
psaJ-rpl33_p2	GTGGCACCAGTGGTAAGTAC	CACTGTTTCGAACACAACTGG	55.0	47.6	58.2	57.9	691-694 bp	1.0	71.5
psaJ-rpl33_p3	TGGCACCAGTGGTAAGTACT	CACTGTTTCGAACACAACTGG	50.0	47.6	58.3	57.9	690-693 bp	1.0	70.9
psaJ-rpl33_p4	TACTCTATGGTTCGGGGCTT	CGGATATCTTTACCCTTGGCC	50.0	52.4	57.8	58.2	630-633 bp	1.0	70.5
psaJ-rpl33_p5	GTGGCACCAGTGGTAAGTAC	CGGATATCTTTACCCTTGGCC	55.0	52.4	58.2	58.2	647-650 bp	1.0	70.3