Hotspot

ndhC-trnV-UAC

LSC · 53443-54028 · 586 bp

Back to Libidibia

Pi 0.0124

MarkerSeek score 69.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 586
variable_sites: 7
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CTGGTCCTTTGCTAATCGGG	TGCACGTTGGGTCTGTAAAA	55.0	45.0	58.3	58.0	799-835 bp	1.0	80.0
ndhC-trnV-UAC_p2	CCTTTGCTAATCGGGGCTAA	TGCACGTTGGGTCTGTAAAA	50.0	45.0	57.7	58.0	794-830 bp	1.0	79.9
ndhC-trnV-UAC_p3	CTGGTCCTTTGCTAATCGGG	AGAAGGTCTACGGTTCGAGT	55.0	50.0	58.3	57.8	734-770 bp	1.0	79.0
ndhC-trnV-UAC_p4	CCTTTGCTAATCGGGGCTAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.7	57.8	729-765 bp	1.0	78.9
ndhC-trnV-UAC_p5	TCCTTTGCTAATCGGGGCTA	TGCACGTTGGGTCTGTAAAA	50.0	45.0	58.5	58.0	795-831 bp	1.0	78.7