Hotspot

trnF-GAA-ndhJ

LSC · 51020-51756 · 737 bp

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Pi 0.0194

MarkerSeek score 72.00

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 737
variable_sites: 14
pi: 0.0194
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	835-841 bp	1.0	81.9
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATCCACGGCTGAAACGTATC	50.0	50.0	58.1	58.1	869-875 bp	1.0	81.8
trnF-GAA-ndhJ_p3	GAGGATGATGCGTCACGAAT	TTGGATAGGATGGCCTTTGC	50.0	50.0	58.1	57.9	895-901 bp	1.0	81.0
trnF-GAA-ndhJ_p4	GAGGATGATGCGTCACGAAT	ATCCACGGCTGAAACGTATC	50.0	50.0	58.1	58.1	929-935 bp	1.0	80.9
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CCACGGCTGAAACGTATCTT	50.0	50.0	58.1	58.3	867-873 bp	1.0	80.1