Hotspot

trnG-UCC-trnfM-CAU

LSC · 38989-39162 · 174 bp

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Pi 0.0287

MarkerSeek score 68.90

Primer available yes

Label trnG-UCC-trnfM-CAU

Feature Metadata

label_name: trnG-UCC-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 5
pi: 0.0287
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnfM-CAU_p1	CTTTGCCAAAGAGAAGACGC	CTTGAGGTCACGGGTTCAAA	50.0	50.0	57.7	58.0	251-255 bp	1.0	78.7
trnG-UCC-trnfM-CAU_p2	CTTTGCCAAAGAGAAGACGC	TGAGGTCACGGGTTCAAATC	50.0	50.0	57.7	57.8	249-253 bp	1.0	77.6
trnG-UCC-trnfM-CAU_p3	CTTTGCCAAAGAGAAGACGC	AGTTTTCTCTTTACCGCGGG	50.0	50.0	57.7	58.2	305-309 bp	1.0	77.2
trnG-UCC-trnfM-CAU_p4	CTTTGCCAAAGAGAAGACGC	GTAGCTCGCAAGGCTCATAA	50.0	50.0	57.7	57.8	272-276 bp	1.0	77.1
trnG-UCC-trnfM-CAU_p5	CTTTGCCAAAGAGAAGACGC	TAGCTCGCAAGGCTCATAAC	50.0	50.0	57.7	57.8	271-275 bp	1.0	77.1