Hotspot

trnS-GCU-trnR-UCU

LSC · 8499-10266 · 1768 bp

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Pi 0.0186

MarkerSeek score 63.00

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1768
variable_sites: 32
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1839-1840 bp	1.0	67.0
trnS-GCU-trnR-UCU_p2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.0	56.9	1838-1839 bp	1.0	67.0
trnS-GCU-trnR-UCU_p3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1845-1846 bp	1.0	66.4
trnS-GCU-trnR-UCU_p4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	58.0	57.9	1844-1845 bp	1.0	66.3
trnS-GCU-trnR-UCU_p5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	50.0	47.6	58.2	56.9	1893-1894 bp	1.0	65.8