Hotspot

trnQ-UUG-psbK

LSC · 6992-7497 · 506 bp

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Pi 0.0211

MarkerSeek score 66.70

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 506
variable_sites: 10
pi: 0.0211
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	TACTCTGGGACGGAAGGATT	AGGCTTCAGGTAATTTTGCGA	50.0	42.9	57.5	58.2	638-670 bp	1.0	71.2
trnQ-UUG-psbK_p2	TACTCTGGGACGGAAGGATT	GGCTTCAGGTAATTTTGCGA	50.0	45.0	57.5	56.7	637-669 bp	1.0	70.5
trnQ-UUG-psbK_p3	TACTCTGGGACGGAAGGATT	AGGCTTCAGGTAATTTTGCG	50.0	45.0	57.5	56.7	638-670 bp	1.0	70.4
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	AGGCTTCAGGTAATTTTGCGA	50.0	42.9	58.7	58.2	600-632 bp	1.0	70.2
trnQ-UUG-psbK_p5	GACCAAAACCCGTTGCCTTA	GGCTTCAGGTAATTTTGCGA	50.0	45.0	58.7	56.7	599-631 bp	1.0	69.5