Hotspot

rpl32-ndhF

SSC · 124174-125135 · 962 bp

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Pi 0.0106

MarkerSeek score 56.80

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 962
variable_sites: 45
pi: 0.0106
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	TTTCCCGTGGAAACCGATTT	ACACATTCCCACAAGTTCCC	45.0	50.0	58.0	58.0	1536-1560 bp	1.0	85.0
rpl32-ndhF_p2	TTTCCCGTGGAAACCGATTT	CACATTCCCACAAGTTCCCA	45.0	50.0	58.0	58.0	1535-1559 bp	1.0	85.0
rpl32-ndhF_p3	TTTCCCGTGGAAACCGATTT	TCGCGTGAACCAAAAACCTA	45.0	45.0	58.0	58.0	1569-1593 bp	1.0	84.5
rpl32-ndhF_p4	TTTCCCGTGGAAACCGATTT	TTTCGTAACTGCACCAACGA	45.0	45.0	58.0	58.1	1785-1809 bp	1.0	83.9
rpl32-ndhF_p5	TTTCCCGTGGAAACCGATTT	AGTGGGAATTGTGCGGATTT	45.0	45.0	58.0	58.1	1802-1826 bp	1.0	83.7