Hotspot

ycf1-rps15

SSC · 113717-114147 · 431 bp

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Pi 0.0175

MarkerSeek score 63.10

Primer available yes

Label ycf1-rps15

Feature Metadata

label_name: ycf1-rps15
feature_type: igs
region: SSC
length_bp: 431
variable_sites: 24
pi: 0.0175
unique_haplotype_count: 11
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-rps15_p1	CCGATAATGGCAATCGTTTCA	ACCTGAAATTCAGCAGACCC	42.9	50.0	57.0	57.8	537-581 bp	0.923077	67.5
ycf1-rps15_p2	ACCGATAATGGCAATCGTTTC	ACCTGAAATTCAGCAGACCC	42.9	50.0	57.0	57.8	538-582 bp	0.923077	67.4
ycf1-rps15_p3	CCGATAATGGCAATCGTTTCA	CCTGAAATTCAGCAGACCCA	42.9	50.0	57.0	57.8	536-580 bp	0.846154	64.1
ycf1-rps15_p4	ACCGATAATGGCAATCGTTTC	CCTGAAATTCAGCAGACCCA	42.9	50.0	57.0	57.8	537-581 bp	0.846154	64.1
ycf1-rps15_p5	CCGATAATGGCAATCGTTTCAG	CCTGAAATTCAGCAGACCCA	45.5	50.0	58.1	57.8	536-580 bp	0.846154	62.8