Hotspot

petA-psbJ

LSC · 62771-63638 · 868 bp

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Pi 0.0157

MarkerSeek score 68.90

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 868
variable_sites: 43
pi: 0.0157
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTACGCGTCCAAGGTCTTTT	GGCTGGGTTCATCTCTGTAG	45.0	55.0	57.8	57.4	760-1046 bp	1.0	80.7
petA-psbJ_p2	CATCCGTTATTTTGGCGCAA	GGCTGGGTTCATCTCTGTAG	45.0	55.0	57.7	57.4	729-1015 bp	1.0	80.5
petA-psbJ_p3	CATTACGCGTCCAAGGTCTT	GGCTGGGTTCATCTCTGTAG	50.0	55.0	58.3	57.4	762-1048 bp	1.0	80.4
petA-psbJ_p4	TTACGCGTCCAAGGTCTTTT	GGGCTGGGTTCATCTCTGTA	45.0	55.0	57.8	58.8	761-1047 bp	1.0	79.2
petA-psbJ_p5	CATCCGTTATTTTGGCGCAA	GGGCTGGGTTCATCTCTGTA	45.0	55.0	57.7	58.8	730-1016 bp	1.0	79.0