Hotspot

ndhC-tRNA-Val

LSC · 50962-51590 · 629 bp

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Pi 0.00781
MarkerSeek score 59.20
Primer available yes
Label ndhC-tRNA-Val

Feature Metadata

label_name
ndhC-tRNA-Val
feature_type
igs
region
LSC
length_bp
629
variable_sites
20
pi
0.00781
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-tRNA-Val_p1 CCGGTCCTTCATTAATCGGA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.1 57.8 776-792 bp 1.0 73.4
ndhC-tRNA-Val_p2 TCCGGTCCTTCATTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.1 57.8 777-793 bp 1.0 73.4
ndhC-tRNA-Val_p3 CCGGTCCTTCATTAATCGGAG AGAAGGTCTACGGTTCGAGT 52.4 50.0 58.2 57.8 776-792 bp 1.0 70.7
ndhC-tRNA-Val_p4 CTCCGGTCCTTCATTAATCGG AGAAGGTCTACGGTTCGAGT 52.4 50.0 58.2 57.8 778-794 bp 1.0 70.7
ndhC-tRNA-Val_p5 CCGGTCCTTCATTAATCGGA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.1 57.5 775-791 bp 1.0 70.7