Hotspot

tRNA-Thr-psbD

LSC · 32236-33259 · 1024 bp

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Pi 0.00482

MarkerSeek score 60.50

Primer available yes

Label tRNA-Thr-psbD

Feature Metadata

label_name: tRNA-Thr-psbD
feature_type: igs
region: LSC
length_bp: 1024
variable_sites: 21
pi: 0.00482
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tRNA-Thr-psbD_p1	GCCCTTTTAACTCAGCGGTA	GGACCAGCCTACAAAAACGA	50.0	50.0	57.9	58.1	1075-1203 bp	1.0	83.8
tRNA-Thr-psbD_p2	GCCCTTTTAACTCAGCGGTA	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.9	58.2	1065-1193 bp	1.0	82.7
tRNA-Thr-psbD_p3	GCCCTTTTAACTCAGCGGTA	CCCTCCGTAACCAATCATCC	50.0	55.0	57.9	57.7	1049-1177 bp	1.0	82.6
tRNA-Thr-psbD_p4	GCCCTTTTAACTCAGCGGTA	TGGCAAATTTACCAAGGGCA	50.0	45.0	57.9	58.3	1001-1129 bp	1.0	82.5
tRNA-Thr-psbD_p5	GGCGTAAGTCATCGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1038-1166 bp	1.0	82.4